I started this RNA project in September, and now that I'm FINALLY ready to do my qPCR (the reaction that will actually give me the results/data), the lab whose machine I was going to borrow has suddenly decided that the machine is old and inaccurate. This is odd since I know they had it serviced in the fall. Oh well. I'm going nuts because I was ready to do this TODAY, and now I'm waiting to hear back on two other leads for machines I could use. I can't find any other on-campus facility that actually offers public use of a qPCR machine, so now I've got to bug individual labs.
Edit: Excellent! As soon as I bitch about this to my LJ, I get an email back with a machine that I can use. That's the way to do it.